: Dimitrios P. Nikolelis, Georgia-Paraskevi Nikoleli
: Biosensors for Security and Bioterrorism Applications
: Springer-Verlag
: 9783319289267
: Advanced Sciences and Technologies for Security Applications
: 1
: CHF 132.90
:
: Sonstiges
: English
: 514
: Wasserzeichen/DRM
: PC/MAC/eReader/Tablet
: PDF
This book offers comprehensive coverage of biomarker/biosensor interactions for the rapid detection of weapons of bioterrorism, as well as current research trends and future developments and applications. It will be useful to researchers in this field who are interested in new developments in the early detection of such. The authors have collected very valuable and, in some aspects indispensable experience in the area i.e. in the development and application of portable biosensors for the detection of potential hazards. Most efforts are centered on the development of immunochemical assays including flow-lateral systems and engineered antibodies and their fragments. In addition, new approaches to the detection of enzyme inhibitors, direct enzymatic and microbial detection of metabolites and nutrients are elaborated. Some realized prototypes and concept devices applicable for the further use as a basis for the cooperation programs are also discussed.
There is a particular focus on electrochemical and optical detection systems,
including those employing carbon nanotubes, quantum dots and metal
nanoparticles The authors are well-known scientists and most of them are editors of respected international scientific journals. Although recently developed biosensors utilize known principles, the biosensing devices described can significantly shorten the time required for successful detection and enhance efforts in more time-consuming direction , e.g. remote sensing systems and validation in real-sample analysis.
The authors describe advances in all stages of biosensor development: the
selection of biochemical components, their use in biosensor assembly, detection principles and improvements and applications for real sample assays.

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Preface6
Contents13
1 Biosensors for Security and Bioterrorism: Definitions, History, Types of Agents, New Trends and Applications16
Abstract16
1 Introduction17
2 Definitions17
3 History18
3.1 20th Century18
3.2 21st Century19
4 Types of Agents19
4.1 Category A19
4.1.1 Anthrax20
4.1.2 Smallpox20
4.1.3 Botulinum Toxin20
4.1.4 Bubonic Plague20
4.1.5 Viral Hemorrhagic Fevers21
4.1.6 Tularemia21
4.2 Category B21
4.3 Category C21
5 Recent Advances of Biosensors for the Rapid Detection of Weapons of Terrorism22
6 Planning and Response23
6.1 Preparedness23
6.2 Aspects of Protection Against Bioterrorism Mainly in the US Include23
6.3 Biosurveillance25
6.4 Response to Bioterrorism Incident or Threat26
7 Conclusions27
References28
2 Microfluidics a Potent Route to Sample Delivery for Non-intrusive Sensors29
Abstract29
1 Introduction30
2 Microfluidics: Basic Features31
3 Dual Flow for Mobile Liquid-Liquid Interfaces31
4 Transport Phenomena Under Flow Conditions34
5 Electrophoretic Field Effects36
6 Cell Resolution37
7 Dual Flow Heterogeneity37
8 In Situ Polymer Membrane Formation39
9 Sensor Incorporation in Microfluidics42
10 Conclusions44
Acknowledgments45
References45
3 New Routes in the High-Throughput Screening of Toxic Proteins Using Immunochemical Tools49
Abstract49
1 Lethal Plant/Bacteria Proteins as Potential Warfare Agents: An Overview49
1.1 Plant Protein Toxins50
1.2 Bacterial Protein Toxins52
2 Nanostructured Immunosensors for the on-Site Detection of Toxic Proteins in Food and Biological Samples54
2.1 Electrochemical Immunosensors59
2.1.1 Amperometric Immunosensors with Highly Conductive Nanoparticle-Modified Transducers59
2.1.2 Impedance Immunosensors62
2.2 Piezoelectric Imunosensors63
2.3 Optical Immunosensors63
2.3.1 Optical Fiber/Fluorescence-Combined Immunoassays64
2.3.2 Surface-Enhanced Raman Scattering (SERS) Imunosensors65
3 Immunoassay Implementation: Labelled Vs. Label-Free Formats for the Detection of Proteins in Liquid Samples66
4 Solving Current Issues in High-Throughput Analysis: Decreasing the Detection Limit and the Response Time of the Sensor66
5 Future Applications in Point-of-Care Systems67
Acknowledgement67
References68
4 Voltammetric Electronic Tongue for the Sensing of Explosives and Its Mixtures74
Abstract74
1 Introduction74
1.1 Detection of Explosives by Simple Voltammetry77
1.2 Improvement of Voltammetric Results with Use of Chemometrics79
2 Systems Using a Single Sensor81
3 Systems Using a Sensor Array86
4 Concluding Remarks92
Acknowledgements92
References93
5 Magneto Actuated Biosensors for Foodborne Pathogens and Infection Diseases Affecting Global Health95
Abstract95
1 Introduction96
1.1 Global Health: A Challenge for Key Enabling Technologies96
1.2 Traditional Methods for the Detection of Food Borne Pathogens and Infection Diseases97
1.3 Technical Challenges in Diagnostic Tests in Low-Resource Settings98
2 Magnetic Particles in ASSURED Diagnostic100
3 Electrochemical Biosensors102
4 Magnetic Immobilisation Coupled with a Magneto-Actuated Electrode for Electrochemical Biosensing105
5 Electrochemical Biosensors of Agents Affecting Food Safety107
5.1 Immuno (IMS) and Phagomagnetic Separation (PMS) Coupled with Electrochemical Genosensing on Magneto Actuated Electrodes108
5.2 Immunomagnetic Separation (IMS) Coupled with Electrochemical Immunosensing on Magneto Actuated Electrodes112
5.3 Simultaneous Electrochemical Magneto Genosensing of Foodborne Bacteria Based on Triple-Tagging Multiplex Amplification113
6 Electrochemical Biosensors of Infection Agents Affecting Global Health113
6.1 Electrochemical Magneto-Actuated Biosensor for CD4 Count in AIDS Diagnosis and Monitoring113
6.2 Electrochemical Magneto-Actuated Biosensor for CD4 Count in AIDS Diagnosis and Monitoring116
7 Final Remarks117
References120
6 Electrochemical Biosensors for Chemical Warfare Agents127
Abstract127
1 Introduction127
2 Nerve Agents130
2.1 Biosensor Based on Cholinesterase Enzyme Inhibition131
2.1.1 Bi-enzymatic