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Advanced Concepts in Fluorescence Sensing Part B: Macromolecular Sensing

:	Chris D. Geddes, Joseph R. Lakowicz
:	Chris D. Geddes, Joseph R. Lakowicz
:	Advanced Concepts in Fluorescence Sensing Part B: Macromolecular Sensing
:	Springer-Verlag
:	9780387236476
:	1
:	CHF 133.60
:

:	Allgemeines, Lexika
:	English

:	300
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology.

Complementary volumes 9& 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.

	CONTRIBUTORS	6
	Preface	8
	Table of Contents	10
	PROTEIN-BASED BIOSENSORS WITHPOLARIZATION TRANSDUCTION	16
	1.1. INTRODUCTION	16
	1.2. PRINCIPLES OF OPERATION	16
	1.3. ADVANTAGES OF ANISOTROPY-BASED SENSING	18
	1.4. FLUORESCENCE POLARIZATION IMMUNOASSAY	19
	1.5. ANISOTROPY-BASED METAL ION BIOSENSING	24
	1.6. ANISOTROPY-BASED SENSING OF OTHERANALYTES USING PROTEINSAS TRANSDUCERS	30
	1.7. CONCLUSIONS	31
	1.8. ACKNOWLEDGMENTS	32
	1.9. REFERENCES	32
	GFP SENSORS	36
	2.1. INTRODUCTION	36
	2.2. GENERAL PRINCIPLES OF ENGINEERING FLUORESCENT PROTEINSENSORS	36
	2.3. NEW GREEN AND RELATED FLUORESCENT PROTEINS	38
	2.3.1. GFP Mutants	38
	2.3.2. Novel Fluorescent Proteins	39
	2.4. GFP-BASED SENSORS	39
	2.4.1. pH Sensors	39
	2.4.2. Chloride/Halide Sensors	41
	2.4.3. Sensors of Protease Activity	43
	2.4.4. Calcium Sensors	43
	2.4.5. Sensors of Calcium-Calmodulin	45
	2.4.6. Sensors of Other Second Messengers	45
	2.4.7. Sensors of Protein Kinase Activity	46
	2.4.8. Sensors of G proteins	47
	2.4.9. Metabolite Sensors	48
	2.4.10. Sensors of Reduction-Oxidation (Redox) Potential	48
	2.4.11. Nitration Sensors	49
	2.4.12. Voltage Sensors	49
	2.5. PERSPECTIVE AND FUTURE DIRECTIONS	50
	2.6. ACKNOWLEDGMENTS	50
	2.7. REFERENCES	51
	FLUORESCENT SACCHARIDE SENSORS	56
	3.1. INTRODUCTION	56
	3.2. READ-OUT	58
	3.2.1. Internal Charge Transfer (ICT)	58
	3.2.2. Photoinduced electron transfer (PET)	60
	3.2.3. Others	61
	3.3. INTERFACE	62
	3.3.1. Internal Charge Transfer (ICT)	62
	3.3.2. Photoinduced electron transfer (PET)	63
	3.3.3. Others	69
	3.4. FLUORESCENT ASSAY	71
	3.5. POLYMER SUPPORTED SENSORS	75
	3.6. CONCLUSIONS	77
	3.7. REFERENCES	77
	FLUORESCENT PEBBLE NANO-SENSORS AND NANOEXPLORERSFOR REAL-TIME INTRACELLULAR AND BIOMEDICAL APPLICATIONS	84
	4.1. INTRODUCTION	84
	4.1.1. Background and History	84
	4.2. PEBBLE MATRICES: DESIGN, PRODUCTION, AND QUALITY CONTROL