| Preface | 6 |
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| Contents | 8 |
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| Editors (and authors): | 14 |
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| 1. Laserneedles in acupuncture | 18 |
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| 1.1 Introduction and motivation | 18 |
| 1.2 Dose-effect relationships in acupuncture | 21 |
| 1.3 Laserneedle acupuncture as a placebo method | 24 |
| 1.4 Physical characteristics of laserneedles | 28 |
| 1.5 Acknowledgements | 33 |
| 1.6 References | 33 |
| 2. Cerebral vascular effects of non invasive laserneedles measured by transorbital and transtemporal Doppler sonography | 35 |
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| 2.1 Introduction | 35 |
| 2.2 Methods | 37 |
| 2.2.1 Non invasive laserneedles | 37 |
| 2.2.2 Multidirectional transorbital and transtemporal Doppler sonography | 39 |
| 2.2.3 Participants | 40 |
| 2.2.4 Acupuncture and procedure | 40 |
| 2.2.5 Statistical Analysis | 42 |
| 2.3 Results | 42 |
| 2.4 Discussion | 44 |
| 2.5 Conclusion | 45 |
| 2.6 Acknowledgements | 46 |
| 2.7 References | 46 |
| 3. Near-infrared spectroscopy for objectifying cerebral effects of needle and laserneedle acupuncture | 49 |
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| 3.1 Introduction | 49 |
| 3.2 Methods | 49 |
| 3.2.1 Near-infrared spectroscopy | 49 |
| 3.2.2 Laserneedle stimulation | 50 |
| 3.2.3 Healthy volunteers, acupuncture, measurement procedure | 51 |
| 3.2.4 Statistical analysis | 53 |
| 3.3 Results | 53 |
| 3.4 Discussion | 55 |
| 3.5 Acknowledgements | 58 |
| 3.6 References | 58 |
| 4. Communication between acupoint and brain proved by ultrasound | 60 |
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| 4.1 Introduction | 60 |
| 4.2 Methods and materials | 60 |
| 4.2.1 TCD monitoring of the anterior and posterior cerebral arteries with a new probe holder | 60 |
| 4.2.2 Participants | 61 |
| 4.2.3 Laserneedle acupuncture | 62 |
| 4.2.4 Statistical Analysis | 65 |
| 4.3 Results | 65 |
| 4.4 Discussion | 70 |
| 4.5 Acknowledgements | 72 |
| 4.6 References | 72 |
| 5. Histological investigations regarding micromorphological effects of laserneedle illumination. Results of an animal experiment | 74 |
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| 5.1 Introduction | 74 |
| 5.2 Methods | 74 |
| 5.2.1 Procedure | 74 |
| 5.2.2 Laserneedle stimulation | 76 |
| 5.2.3 Laser Doppler flowmetry and temperature measurement | 76 |
| 5.3 Results | 77 |
| 5.4 Discussion | 78 |
| 5.5 Acknowledgements | 79 |
| 5.6 References | 79 |
| 6. Effects of laserneedle stimulation on microcirculation and skin temperature | 81 |
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| 6.1 Introduction | 81 |
| 6.2 Method and volunteers | 81 |
| 6.2.1 Laser Doppler flowmetry | 81 |
| 6.2.2 Volunteers and procedure | 82 |
| 6.2.3 Statistical analysis | 84 |
| 6.3 Results | 84 |
| 6.4 Discussion | 87 |
| 6.5 References | 89 |
| 7. Effects of acupressure, manual acupuncture and laserneedle acupuncture on EEG bispectral index (BIS) and spectral edge frequency (SEF) in healthy volunteers | 90 |
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| 7.1 Introduction | 90 |
| 7.2 Material and methods | 90 |
| 7.2.1 Subjects | 90 |
| 7.2.2 Procedure and study design | 91 |
| 7.2.3 Evaluation parameters | 93 |
| 7.2.4 Statistical analysis | 94 |
| 7.3 Results | 94 |
| a b c d e f g | 96 |
| 7.4 Discussion | 98 |
| 7.5 Acknowledgements | 102 |
| 7.6 References | 102 |
| 8. Acupuncture using laserneedles modulates brain function: evidence from functional transcranial Doppler sonography (fTCD) and functional magnetic resonance imaging (fMRI) | 104 |
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| 8.1 Introduction | 104 |
| 8.2 Methods | 105 |
| 8.2.1 Painless laserneedles | 105 |
| 8.2.2 Functional multidirectional transcranial Doppler sonography (fTCD) | 105 |
| 8.2.3 Functional magnetic resonance imaging (fMRI) | 106 |
| 8.2.4 Participants | 106 |
| 8.2.5 Experimental design and procedure | 106 |
| 8.2.6 Evaluated parameters | 108 |
| 8.2.7 Statistical analysis | 109 |
| 8.3 Results | 109 |
| 8.4 Discussion | 113 |
| 8.5 Conclusions | 115 |
| 8.6 Acknowledgements | 115 |
| 8.7 References | 115 |
| 9. Quantification of gender specific thermal sensory and pain thresholds before and after laserneedle stimulation | 118 |
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| 9.1 Introduction | 118 |
| 9.2 Method | 118 |
| 9.2.1 Volunteers | 118 |
| 9.2.2 Measurement of thermal sensitivity and thermal pain thresholds | 119 |
| 9.2.3 Laserneedle acupuncture and procedure | 120 |
| 9.2.4 Statistical analysis | 122 |
| 9.3 Results | 122 |
| 9.4 Discussion | 126 |
| 9.5 Acknowledgements | 127 |
| 9.6 References | 127 |
| 10. Biological effects of painless laserneedle acupuncture - a short summary of important scientific results | 129 |
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| 10.1 Introduction | 129 |
| 10.2 Methods | 129 |
| 10.2.1 Temperature and microcirculatory monitoring | 129 |
| 10.2.2 Functional multi-directional transcranial Doppler-sonography (fTCD) | 130 |
| 10.2.3 Functional magnetic resonance imaging (fMRI) | 130 |
| 10.2.4 Near infrared spectroscopy (NIRS) | 130 |
| 10.2.5 Laserneedle stimulation | 130 |
| 10.2.6 Healthy volunteers, animal experiment and procedures | 131 |
| 10.2.7 Statistical analysis | 131 |
| 10.2.8 Evaluation parameters | 132 |
| 10.3 Results | 132 |
| 10.4 Discussion | 138 |
| 10.5 Conclusion | 139 |
| 10.6 Acknowledgements | 139 |
| 10.7 References | 139 |
| 11. Increases in intracranial pressure and changes in blood flow velocity due to acupressure, needle and laserneedle acupuncture? | 141 |
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| 11.1 Introduction | 141 |
| 11.2 Material and methods | 141 |
| 11.2.1 Volunteers, patient, acupressure and procedure | 141 |
| 11.2.2 Measurement techniques, evaluation parameters and statistical analysis | 143 |
| 11.3 Results | 144 |
| 11.4 Discussion | 148 |
| 11.5 Acknowledgements | 150 |
| 11.6 References | 150 |
| 12. Laserneedle acupuncture - clinical studies | 152 |
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| 12.1 Introduction | 152 |
| 12.2 Material, patients and methods | 153 |
| 12.3 Results | 156 |
| 12.3.1 Lumbar, thoracic and cervical spine syndromes
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