| 112284_1_En_FM1_OnlinePDF | 1 |
|---|
| 112284_1_En_1_Chapter_OnlinePDF | 9 |
|---|
| 1: Comparative Genomic Analysis of Pathogenic Yeasts and the Evolution of Virulence | 9 |
| Comparative Genome Analysis | 11 |
| Analysis of Basidiomycete Genomes | 12 |
| Analysis of Ascomycete Genomes | 13 |
| The Aspergilli | 13 |
| Candida Genomes | 14 |
| Evolution of Gene Families Associated with Virulence | 15 |
| Analysis of Synteny in Candida Genomes | 17 |
| Differential Gene Regulation and Evolution of Virulence | 20 |
| References | 22 |
| 112284_1_En_2_Chapter_OnlinePDF | 27 |
|---|
| Chapter 2: Molecular Epidemiology of Candida Species | 27 |
| Introduction | 27 |
| Epidemiology of Candidosis | 28 |
| Molecular Epidemiology | 30 |
| DNA Fingerprinting with Dispersed, Repetitive Elements | 30 |
| Multi-Locus Sequence Typing (MLST) of C. albicans | 32 |
| Defining Clonal Clusters by MLST | 35 |
| Insights on Population Structure of C. albicans from MLST | 35 |
| Epidemiological Investigations with MLST | 38 |
| MLST of Non-C. albicans Candida Species | 39 |
| Future Directions for Typing of Candida Species | 41 |
| Conclusions | 42 |
| References | 43 |
| 112284_1_En_3_Chapter_OnlinePDF | 48 |
|---|
| Chapter 3: Candida Infections and Modelling Disease | 48 |
| Candida Species as Commensals | 48 |
| Candida Species Associated with Disease | 50 |
| Oral Candidiasis | 50 |
| Vaginal Candidiasis | 51 |
| Disseminated Candidiasis | 52 |
| Modelling Candida Carriage and Infection | 54 |
| Experimental Models of Candida Infection: The Reductionist Approach | 54 |
| Cell-Based Models | 54 |
| Monocyte-Candida Interactions | 54 |
| Macrophage-Candida Interactions | 55 |
| Neutrophil-Candida Interactions | 56 |
| Dendritic Cell-Candida Interactions | 56 |
| Epithelial Cell- and Endothelial Cell-Candida Interactions | 57 |
| Reconstituted Human Epithelial Models (RHE) and Tissue Explants | 57 |
| Whole Animal Approaches | 58 |
| Invertebrate Models | 58 |
| Vertebrate Models | 58 |
| Mucosal Models | 59 |
| Models of Oral and Gastrointestinal Carriage and Infection | 59 |
| Models of Vaginal Carriage and Infection | 60 |
| Models of Systemic Infection | 61 |
| Gastrointestinal Dissemination | 61 |
| Intravenous Challenge | 61 |
| References | 62 |
| 112284_1_En_4_Chapter_OnlinePDF | 75 |
|---|
| Chapter 4: Candida albicans Cell Wall Mediated Virulence | 75 |
| C. albicans Cell Wall Architecture | 75 |
| Cell Wall Proteins | 76 |
| Cell Wall Remodelling During Morphogenesis and Variation in pH | 78 |
| Phenotypic Switching | 81 |
| Comparative Cell Wall-Omics | 81 |
| Glycosylphosphatidylinositol Anchored Cell Wall Proteins | 82 |
| Cell Wall Associated Virulence Attributes | 82 |
| Adhesion | 82 |
| Als family | 83 |
| Eap1 | 84 |
| Hwp1 | 84 |
| Ywp1 | 84 |
| Int1 | 85 |
| Cell Surface Hydrophobicity | 85 |
| Superoxide Dismutases (SODs) | 86 |
| Phospholipases | 86 |
| Iron Binding Proteins | 87 |
| Als3 a Multi-Functional Adhesin, Invasin and Ferritin-Binding Protein | 87 |
| Biofilm Formation | 88 |
| Interactions with Host Cells | 89 |
| Wall as Potential Source of Novel Therapies | 90 |
| Future Perspectives | 90 |
| References | 91 |
| 112284_1_En_5_Chapter_OnlinePDF | 102 |
|---|
| Chapter 5: Secreted Candida Proteins: Pathogenicitypathogenicity and Host Immunity | 102 |
| Introduction | 102 |
| The Secretory Pathwaysecretory pathway in Fungi | 103 |
| The Secretory Proteins of Candida spp. | 104 |
| Non-Covalently Linked Cell Wall Associated Secreted Proteins | 104 |
| Candida Proteins Secreted from the Cell | 107 |
| Candida Secreted Aspartyl Proteinases | 107 |
| Candida Secreted Aspartyl Proteinases: Processing, Activation and Structuresecreted aspartyl proteinasesprocessing, activation | 108 |
| Candida Secreted Aspartyl Proteinases: Role in Pathogenicitysecreted aspartyl proteinasesrole in pathogenicity | 109 |
| Candida Secreted Phospholipases | 113 |
| Candida Phospholipases: Role in Pathogenicityphospholipasesrole in pathogenicity | 114 |
| Candida-Secreted Lipases | 114 |
| Candida Lipases: Role in Pathogenicitylipasesrole in pathogenicity | 115 |