: Ruth Ashbee, Elaine M. Bignell
: Ruth Ashbee, Elaine M Bignell
: Pathogenic Yeasts
: Springer-Verlag
: 9783642031502
: 1
: CHF 190.00
:
: Mikrobiologie
: English
: 365
: Wasserzeichen
: PC/MAC/eReader/Tablet
: PDF

Mycological studies of yeasts are entering a new phase, with the sequencing of multiple fungal genomes informing our understanding of their ability to cause disease and interact with the host. At the same time, the ongoing use of traditional methods in many clinical mycology laboratories continues to provide information for the diagnosis and treatment of patients. This volume reviews various aspects of pathogenic yeasts and what is known about their molecular and cellular biology and virulence, in addition to looking at clinical and laboratory findings. As each chapter is written by a leading expert in the field, this book summarizes in one volume much of the latest research on several pathogenic yeasts, including Candida, Cryptococcus, Malassezia and yeasts of emerging importance. The importance of laboratory diagnosis, antifungal susceptibility testing, antifungal resistance and yeast diseases in animals are reviewed.

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1: Comparative Genomic Analysis of Pathogenic Yeasts and the Evolution of Virulence9
Comparative Genome Analysis11
Analysis of Basidiomycete Genomes12
Analysis of Ascomycete Genomes13
The Aspergilli13
Candida Genomes14
Evolution of Gene Families Associated with Virulence15
Analysis of Synteny in Candida Genomes17
Differential Gene Regulation and Evolution of Virulence20
References22
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Chapter 2: Molecular Epidemiology of Candida Species27
Introduction27
Epidemiology of Candidosis28
Molecular Epidemiology30
DNA Fingerprinting with Dispersed, Repetitive Elements30
Multi-Locus Sequence Typing (MLST) of C. albicans32
Defining Clonal Clusters by MLST35
Insights on Population Structure of C. albicans from MLST35
Epidemiological Investigations with MLST38
MLST of Non-C. albicans Candida Species39
Future Directions for Typing of Candida Species41
Conclusions42
References43
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Chapter 3: Candida Infections and Modelling Disease48
Candida Species as Commensals48
Candida Species Associated with Disease50
Oral Candidiasis50
Vaginal Candidiasis51
Disseminated Candidiasis52
Modelling Candida Carriage and Infection54
Experimental Models of Candida Infection: The Reductionist Approach54
Cell-Based Models54
Monocyte-Candida Interactions54
Macrophage-Candida Interactions55
Neutrophil-Candida Interactions56
Dendritic Cell-Candida Interactions56
Epithelial Cell- and Endothelial Cell-Candida Interactions57
Reconstituted Human Epithelial Models (RHE) and Tissue Explants57
Whole Animal Approaches58
Invertebrate Models58
Vertebrate Models58
Mucosal Models59
Models of Oral and Gastrointestinal Carriage and Infection59
Models of Vaginal Carriage and Infection60
Models of Systemic Infection61
Gastrointestinal Dissemination61
Intravenous Challenge61
References62
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Chapter 4: Candida albicans Cell Wall Mediated Virulence75
C. albicans Cell Wall Architecture75
Cell Wall Proteins76
Cell Wall Remodelling During Morphogenesis and Variation in pH78
Phenotypic Switching81
Comparative Cell Wall-Omics81
Glycosylphosphatidylinositol Anchored Cell Wall Proteins82
Cell Wall Associated Virulence Attributes82
Adhesion82
Als family83
Eap184
Hwp184
Ywp184
Int185
Cell Surface Hydrophobicity85
Superoxide Dismutases (SODs)86
Phospholipases86
Iron Binding Proteins87
Als3 a Multi-Functional Adhesin, Invasin and Ferritin-Binding Protein87
Biofilm Formation88
Interactions with Host Cells89
Wall as Potential Source of Novel Therapies90
Future Perspectives90
References91
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Chapter 5: Secreted Candida Proteins: Pathogenicitypathogenicity and Host Immunity102
Introduction102
The Secretory Pathwaysecretory pathway in Fungi103
The Secretory Proteins of Candida spp.104
Non-Covalently Linked Cell Wall Associated Secreted Proteins104
Candida Proteins Secreted from the Cell107
Candida Secreted Aspartyl Proteinases107
Candida Secreted Aspartyl Proteinases: Processing, Activation and Structuresecreted aspartyl proteinasesprocessing, activation108
Candida Secreted Aspartyl Proteinases: Role in Pathogenicitysecreted aspartyl proteinasesrole in pathogenicity109
Candida Secreted Phospholipases113
Candida Phospholipases: Role in Pathogenicityphospholipasesrole in pathogenicity114
Candida-Secreted Lipases114
Candida Lipases: Role in Pathogenicitylipasesrole in pathogenicity115