ebook ebooks e-book e-books downloaden bei MyEbooks.ch downloaden

Insertional Mutagenesis Strategies in Cancer Genetics

:	Adam J. Dupuy, David A. Largaespada
:	Adam J. Dupuy, David A. Largaespada
:	Insertional Mutagenesis Strategies in Cancer Genetics
:	Springer-Verlag
:	9781441976567
:	1
:	CHF 85.20
:

:	Nichtklinische Fächer
:	English

:	200
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

The goal of this work is summarize the contribution that insertional mutagenesis has made to our understanding of cancer. A variety of insertional mutagens are presented that have been used to study a variety of tumor types in several model organisms. In addition, the impact of insertional mutagenesis in several gene therapy trials is discussed along with strategies to avoid such complications in future clinical trials.

Adam Dupuy, Ph.D., is an Assistant Professor at the Carver College of Medicine at the University of Iowa, and a rising star in the field of insertional mutagenesis; two of the last of his publications have been in Nature, and he was the lead author on one of these. Dr. Dupuy earned his B.A. at Central College in Pella, Iowar, went on to earn his Ph.D. at the University of Minnesota in the Program in Molecular, Cellular, Developmental Biology& Genetics, and did his Postdoctoral fellowship in the Mouse Cancer Genetics Program at the NCI-Frederick.

	Contents	5
	Contributors	6
	1 Insertional Mutagenesis: A Powerful Tool in Cancer Research	8
	1.1 Introduction	8
	1.2 Historical Perspective	9
	1.3 The Site of Insertion can Provide Mechanistic Insights	11
	1.4 Methodologies	12
	1.5 Pairing Insertional Mutagenesis with Other Genetic Tools	15
	1.6 Mining Hidden Information in Mutagenesis Screens	17
	1.7 Importance of Thorough Bioinformatics Analysis	19
	1.8 The Added Value of Large Numbers	20
	1.9 Crossvalidation with Other Genetic Datasets	20
	1.10 Are New Cancer Causing Pathways to be Found	21
	1.11 Conclusions and Recommendations	22
	References	23
	2 Retroviral Insertional Mutagenesis in Mouse Models of Leukemia and Lymphoma	26
	2.1 Introduction to the Murine Leukemia Viruses	26
	2.2 Viral and Host Determinants of Disease	28
	2.3 Challenges in MLV Mutagenesis Studies	30
	2.4 Comparative Oncogenomics	36
	2.5 Validation of Candidates	38
	2.6 Do Murine Retroviruses Cause Human Cancer	40
	2.7 Summary and Future Perspectives	40
	References	41
	3 Gene Discovery by MMTV Mediated Insertional Mutagenesis	46
	3.1 Introduction	46
	3.1.1 Mouse Mammary Gland Biology	48
	3.1.2 Mouse Mammary Tumor Virus	48
	3.1.3 Viral Genes and Genomic Organization	50
	3.1.4 Transcription and Translation of the Viral Genes	51
	3.1.5 Virion Structure and Assembly	52
	3.1.6 Infectious Life Cycle	53
	3.1.7 Tissue Specificity and Hormonal Regulation of MMTV Replication	54
	3.1.8 Exogenous MMTV	55
	3.1.9 Endogenous MMTV	56
	3.1.10 MMTV-Induced Tumors	56
	3.1.11 MMTV Induced Hormone Dependent Premalignant Lesions	57
	3.1.12 Hormone-Dependent Mammary Tumors and MMTV	58
	3.1.13 MMTV-Induced Tumors in other Tissues	58
	3.2 Gene Discovery by MMTV Mediated Insertional Mutagenesis	59
	3.2.1 Common Integration Sites	59
	3.2.2 Modes by Which Insertional Mutagenesis Affects Cancer Genes	60
	3.3