| Chapter 1: Developmental Biology of Somatic Embryogenesis | 22 |
|---|
| Introduction | 22 |
| Basic Requirements for In Vitro SE | 23 |
| Explant and Stem Cell Biology | 24 |
| Genotype | 24 |
| Explant Cells | 25 |
| Earliest Event in Embryogenesis-Asymmetric Cell Division | 27 |
| Cell Wall in Establishment of Polarity, Division Asymmetry and Cell Fate | 27 |
| Division Asymmetry in the Initiation of SE | 29 |
| Asymmetric Division and the Suspensor in SE | 29 |
| Stress Component in the Initiation of SE | 30 |
| Reactive Oxygen Species | 30 |
| Stress-Related Hormone Signalling | 31 |
| Hormones and the Initiation of SE | 32 |
| Induction of SE by Over-Expression of Leafy Cotyledon Transcription Factors and Their Relationship to SE Induction and Repress | 33 |
| ABA, Stress and GA | 35 |
| Soluble Signals and Cell-Cell Interactions that Promote SE in Suspension Cultures | 35 |
| Secreted Proteins that Influence SE | 35 |
| AGP Signalling in SE: Mechanisms and Interactions Between Signalling Pathways | 36 |
| Cell-Cell Interaction and Relevance to SE in Suspension Cultures | 37 |
| Development Program After SE Induction | 38 |
| Concluding Remarks and a Model Based on Studies in Medicago truncatula | 38 |
| SE and Biotechnology | 39 |
| References | 40 |
| Chapter 2: Microspore Embryogenesis | 46 |
|---|
| Introduction | 46 |
| Discovery of the Production of Haploids by Anther Cultureanther culture | 48 |
| Strategies for the Induction of Microspore Embryogenesis | 48 |
| Influence of Different Factors in Microspore Embryogenesis | 49 |
| Genotype | 49 |
| Donor Plant Physiology | 50 |
| Stage of Pollen Development | 50 |
| Pre-treatments | 51 |
| Culture Conditions | 51 |
| Composition of the Medium | 52 |
| Cellular and Molecular Events Associated with Microspore Embryogenesis | 53 |
| Embryogenic Induction | 53 |
| Early Embryogenic Divisions | 56 |
| Development of Embryo Pattern | 57 |
| Plant Formation and Diploidization | 58 |
| Conclusions | 58 |
| References | 59 |
| Chapter 3: Stress and Somaclonal Variation | 64 |
|---|
| Introduction | 64 |
| Stress Responses in Plants | 65 |
| Short-Term Responses | 65 |
| Long-Term Responses | 66 |
| Modifications Induced by Stress Could be Inheritable | 68 |
| Tissue Culture Imposes a Stress to Cultivated In Vitro Cells | 69 |
| Cultured Cellscultured cells and Regenerated Plantsregenerated plants Show Variations | 71 |
| Heritable Changes Versus Non-Heritable Changes | 73 |
| Genetic Versus Epigenetic Changes | 74 |
| Variation Promoted by Tissue Culture is Not Randomly Distributed in the Genome | 76 |
| Are New Alleles Originated by In Vitro Stress Already Present in Other Plants of In Vivo Populations? | 77 |
| Concluding Remarks | 77 |
| References | 77 |
| Chapter 4: Photosynthate Partitioning | 85 |
|---|
| Introduction | 85 |
| Source and Sink | 87 |
| Sugars as Signalling Molecules | 87 |
| Key Metabolic Regulators | 88 |
| SNF1-Related Protein Kinase 1 (SnRK1) | 88 |
| Hexokinase | 92 |
| The Trehalose Pathway | 92 |
| Applications in Biotechnology | 93 |
| Concluding Remarks | 97 |
| References | 98 |
| Chapter 5: Molecular Physiology of Seed Maturation and Seed Storage ProteinSeed Storage Protein Biosynthesis | 101 |
|---|
| Introduction | 101 |
| Seed MaturationSeed maturation | 102 |
| SucroseSucrose as a Maturation Signal | 103 |
| Synthesis and Deposition of Storage Proteins in Crop Seedscrop seeds | 104 |
| Storage Proteins in Cereals | 105 |
| Storage Proteins in the Different Grain Parts | 105 |
| Transcriptional RegulationTranscriptional regulation of Arabidopsis Seed MaturationArabidopsis seed maturation | 107 |
| Transcriptional Regulation of Arabidopsis Seed Maturation-a Model Also for Cereal Seeds? | 107 |
| Unravelling Transcriptional Regulation by Co-Expression Analysisco-expression analysis | 108 |
| DNA MethylationDNA methylation and Storage Protein Gene Expression in the Barley Endosperm | 110 |
| Metabolic ControlMetabolic control of Seed Storage Protein Synthesis | 111 |
| Nitrogen Availability and Signalling | 111 |
| Nitrogen TransportNitrogen transport into Seeds | 112 |
| Overexpression of an Amino Acid Transporteramino acid transporter in Legume Seeds | 112 |
| Carbon Availability | 114 |
| Response to Increased Nitrogen to Carbon Statuscarbon status | 114 |
| Overexpression of Phosphoenolpyruvate Carboxylasephosphoenolpyruvate carboxylase in Narbon Beans | 114 |
| Repression of ADP-Glucose PyrophosphorylaseADP-glucose pyrophosphorylase in Narbon Beans | 115 |
| Repression of ADP-Glucose Pyrophosphorylase in Pea | 116 |
| Outlook | 116 |
| References | 117 |
| Chapter 6: Fatty Acid Biosynthesis and Regulation in Plants | 123 |
|---|
| Introduction | 123 |
| Fatty Acid Biosynthesis | 124 |
| De Novo Fatty Acid Biosynthesis | 124 |
| Regulation of ACCase | 126 |
| Fatty Acid Elongation | 127 |
| Fatty Acid Desaturation | 128 |
| Unusual Fatty Acids | 128 |
| Assembly of Fatty Acids | 129 |
| Conclusions | 130 |
| References | 130 |
| Chapter 7: Biosynthesis and Regulation of Carotenoids in Plants-Micronutrients, Vitamins and Health Benefits | 1
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