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Mesenchymal Stem Cells for the Heart From Bench to Bedside

:	Jian-an Wang
:	Mesenchymal Stem Cells for the Heart From Bench to Bedside
:	Springer-Verlag
:	9783540881506
:	Advanced Topics in Science and Technology in China
:	1
:	CHF 85.50
:

:	Medizin
:	English

:	113
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

Stem cell research has the potential to affect the lives of millions of people around the world. This research is now regularly front-page news, and realizing the promise of mesenchymal stem cells for yielding new medical therapies will require us to grapple with more than just scientific uncertainties. 'Mesenchymal Stem Cells for the Heart - From Bench to Bedside' presents the cytobiological characteristics of mesenchymal stem cells from the isolation, culture, transmembrane ion currents, migration and differentiation in vitro to the repairing of injured myocardium and tissue reconstruction in vivo, including the results of basic research and the real possibility for treatments and ultimately for cures for cardiac diseases for which adequate therapies do not exist. The book is intended for clinical stem cell researchers in cardiovasology, hematology, cytobiology, molecular biology, cell and tissue engineering, and other related fields.

Jian-an Wang, MD, PhD, Professor of Cardiology at Zhejiang University and investigator at Loma Linda University, Southern California, has broad expertise in the molecular and cellular biological processes underlying cardiovascular disease. He has won the Chinese Medical Science and Technology Medal of Honor.

Jian-an Wang, MD, PhD, Professor of Cardiology at Zhejiang University and investigator at Loma Linda University, U.S., has broad expertise in the molecular and cellular biological processes underlying cardiovascular disease. He has won the Chinese Medical Science and Technology Medal of Honor. He performs cardiac interventional treatment on more than 1000 cases per year, doing percutaneous coronary angiography (PTCA), percutaneous coronary angioplasty, radiofrequency catheterization ablation (RFCA), percutaneous closure of a residual ventricular septal defect (VSD), atrial septal defect (ASD) and patent ductus arteriosus (PDA) with the Amplatzer occluder, etc. He is also the chief editor of Electrocardiology Journal, Chinese editor of Circulation, European Heart Journal and American Heart Association Journals Best Selection, editor of Chinese Emergency Journal, Chinese Practical Medicine Journal, and Chinese Modern Clinical Medicine Journal.

	Preface	6
	Contents	7
	MSCs Isolation and Culture Ex Vivo	10
	1.1 Cell Isolation	11
	1.1.1 Direct Adherence Method 1.1.1.1 Materials	12
	1.1.1.2 Procedures	12
	1.1.2 Density Gradient Centrifugation	12
	1.1.2.1 Materials	13
	1.1.2.2 Procedures	14
	1.1.3 Magnetic Microbead or Flow Cytometry Method	15
	1.2 Cell Culture	16
	1.2.1 Materials	16
	1.2.2 Procedures	16
	1.3 Cell Cryopreservation and Thawing	17
	1.3.1 Materials	18
	1.3.2 Procedures	18
	References	18
	2 MSCs Identification	21
	Xinyang Hu1	21
	Abstract:	21
	2.1 Minimal Criteria	22
	2.2 Some Comments of the Criteria	22
	2.3 Other Methods	23
	2.3.1 Morphology characteristics	23
	2.3.2 Other Markers 2.3.2.1 SB- 10	23
	2.3.2.2 SH-2, SH-3, SH-4	23
	2.3.2.3 Neural Ganglioside GD2	24
	2.3.2.4 SSEA-4	24
	2.4 Conclusion	24
	References	25
	3 Biological Characteristics of MSCs	26
	3.1 Surface Markers and Paracrine Characteristics	26
	3.1.1 Surface Markers	26
	3.1.2 Paracrine Characteristics of MSCs	29
	3.2 Electrophysiological Properties of MSCs and Their Electric Coupling with Cardiomyocytes	30
	3.2.1 Characterization of Ion Channels in MSCs	30
	3.2.1.1 Potassium Channels (K+ Channels)	31
	3.2.1.2 Calcium Channels (Ca2+ Channels)	32
	3.2.1.3 Sodium Channels (Na+ Channels)	33
	3.2.2 Why does an Individual Cell Express Different Currents?	33
	3.2.3 Electric Coupling of MSCs with Host Cardiomyocytes	34
	3.3 Proliferation of MSCs and Telomerase Properties	38
	3.3.1 Structure and Function of Telomeres and Telomerase	39
	3.3.2 Telomere and Telomerase in MSCs	39
	3.4 Multilineage Transdifferentiation of MSCs	41
	3.4.1 MSCs and Cardiomyogenesis	42
	3.4.2 Differentiation Fate of MSCs	45
	3.5 Immunological Characteristics of MSCs	46
	3.5.1 Immunological Characteristics of MSCs	46
	3.5.1.1 MSCs and T Lymphocytes	47
	3.5.1.2 MSCs and B Cells	49
	3.5.1.3 MSCs and APCs	50
	3.5.1.4 MSCs and NK Cells	51
	3.5.2	51
	Studies	51
	3.5.3 Immune-related Properties of MSCs in Cardiology	53
	3.6 Conclusion	53
	References	54
	4 Utilization of MSCs for Repairing Cardiomyocytes	66
	4.1 Application of MSCs on Myocardial Infarction	68
	4.2 Application of MSCs on Cardiomyopathy and Chronic Heart Failure	72
	4.3 Conclusion	74
	References	75
	5 Current Status of MSCs in Clinical Application	80
	5.1 The Type and Number of Transplanted Cells	86
	5.2 Cell Preparation	87
	5.3 The Time Point and Pathway of Cell Transplantation	87
	5.4 Others	88
	5.5 Conclusion	90
	References	90
	6 MSCs as a Vector of Gene Engineering	94
	6.1 Adenoviruses and Angiogenesis-related Genes	96
	6.2 Adeno-associated Virus (AAV) and Anti- inflammation Related Genes	97
	6.3 Retrovirus and Anti-apoptosis Related Genes	98
	6.4 Lentivirus and Pacemaker Current Gene	99
	6.5 Conclusion	100
	References	100
	7 Feasibility of MSCs Transplantaion	103
	7.1 General Ethical Considerations	104
	7.2 Safety Issues	104
	7.3 Informed Consent	106
	7.4 Conclusion	106
	References	107
	8 Status and Expectation of MSCs Therapy	108
	8.1 Clinical Application and Outcomes	109
	8.2 Mechanism of Therapeutic Effects	111
	8.3 Expectations with MSCs Therapy	112
	References	114
	Index	118