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Rabbit Biotechnology Rabbit genomics, transgenesis, cloning and models

:	Louis-Marie Houdebine, Jianglin Fan
:	Louis-Marie Houdebine, Jianglin Fan
:	Rabbit Biotechnology Rabbit genomics, transgenesis, cloning and models
:	Springer-Verlag
:	9789048122271
:	1
:	CHF 132.90
:

:	Nichtklinische Fächer
:	English

:	136
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

Louis-Marie Houdebine and Jianglin Fan The study of biological functions of proteins and their possible roles in the pathogenesis of human diseases requires more and more relevant animal m- els. Although mice including genetically modified mice offer many possibilities, other non-murine species are absolutely required in some circumstances. Rabbit is one of these species, which has been widely used in biomedical studies. This animal is genetically and physiologically closer to humans including cardiov- cular system and metabolism characteristics. Rabbit is thus more appropriate than mice to study some diseases such as atherosclerosis and lipid metabolism. Because of its larger size, surgery manipulation, bleeding, and turn-over studies are much easier performed in rabbits than in mice. Furthermore, transgenic rabbits can be produced using microinjection and other methods such as lentiviral v- tors. Cloning in rabbits has been proved possible, even though still laborious and time-consuming. Hopefully, functional rabbit ES cell lines will be available in the coming years. Gene deletion or knock-out in rabbits will then become possible.

	Contents	5
	Chapter 1 Introduction	9
	Chapter 2 Improvement of Rabbit Production	11
	2.1 Introduction	11
	2.2 Artificial Insemination (AI)	12
	2.3 Induction of Parturition by Oxytocin	16
	2.4 Nursing of Suckling Pups	16
	2.5 Storage of Rabbit Semen	17
	2.6 Rabbit Semen Freezing	17
	References	19
	Chapter 3 Basic Methods for Experimental Rabbits	21
	3.1 Introduction	21
	3.2 Handling and Restraint	21
	3.3 Compound Administration	24
	3.4 Sampling Techniques	27
	3.5 Anesthesiology	29
	3.6 Euthanasia	30
	Reference	30
	Chapter 4 Useful Information for Rabbit Genes, Proteins, and Antibodies	31
	4.1 Introduction	31
	4.2 Rabbit Genome, cDNA, and Protein Sequences	31
	4.3 Primary Antibodies Against Rabbit Proteins	32
	4.4 Other Useful Websites for Rabbit Physiology	34
	Chapter 5 General Physiology of Rabbits	35
	5.1 Delivering and Nursing Physiology (Tables 5.1–5.3)	35
	5.2 Behavioral and Sensory Physiology	37
	5.3 Urinary Physiology (Table 5.4)	37
	5.4 Digestive Physiology (Table 5.5)	38
	5.5 Respiratory Physiology	38
	5.6 Cardiac Physiology (Table 5.6)	39
	5.7 Hematologic Values	39
	5.8 Serum Biochemical and Enzyme Values	39
	5.9 Bleeding System for Obtaining More Correct Physiological Data and Animal Welfare	40
	References	42
	Chapter 6 Rabbit Transgenesis	44
	6.1 Introduction	44
	6.2 Pronuclear Microinjection Methods for Rabbit Transgenesis	45
	6.3 Basic Technique for Microinjection	45
	6.4 Other Possible Future Methods for Rabbit Transgenesis	50
	References	52
	Chapter 7 Rabbit as a Model for the Study of Human Diseases	55
	7.1 Introduction	55
	7.2 Studies Using Rabbit Models	56
	7.3 Rabbit Models for Lipid Metabolism and Atherosclerosis	57
	7.4 Rabbit Models for Other Human Diseases	61
	7.5 Conclusions	65
	References	66
	Chapter 8 Transgenic Rabbits to Prepare Pharmaceutical Proteins	70
	8.1 Introduction	70
	8.2 Techniques to Generate Transgenic Rabbits	71
	8.3 Production in Blood	72
	8.4 Production in Milk	73
	8.5 Quality of the Proteins Secreted in Milk	74
	8.6 The Specific Properties of Rabbits as Living Fermentor	77
	8.7 Conclusion and Perspectives	77
	References	78
	Chapter 9 Derivation and Characterization of Rabbit Embryonic Stem Cells: A Review	81
	9.1 Introduction	81
	9.2 Pluripotent Stem Cells of Different Origins	82
	9.3 Rabbit Embryonic Stem Cells	84
	9.4 Discussion	103
	References	103
	Chapter 10 Rabbit Cloning	109
	10.1 Historical Background of Rabbit Biotechnology	109
	10.2 Special Aspects of Rabbit Reproduction	110
	10.3 State of Nuclear Transfer	112
	10.4 Possible Applications of Rabbit Cloning	120
	10.5 Appendix: Protocol for Rabbit Somatic-Cell Nuclear Transfer	122
	Bibliography	124
	Chapter 11 The European Rabbit Genome	133
	Index	134