: Barbara M. Reed
: Barbara B.M. Reed
: Plant Cryopreservation: A Practical Guide A Practical Guide
: Springer-Verlag
: 9780387722764
: 1
: CHF 140.40
:
: "Landwirtschaft, Gartenbau; Forstwirtschaft, Fischerei, Ernährung"
: English
: 514
: Wasserzeichen/DRM
: PC/MAC/eReader/Tablet
: PDF

Cryoprese vation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.

Preface6
Acknowledgements8
Contents9
Contributors19
Section I: Introduction to Cryopreservation28
Cryopreservation—Practical Considerations29
Cryopreservation Theory40
Development of PVS-Based Vitrification and Encapsulation– Vitrification Protocols58
The Development of Encapsulation Dehydration83
Controlled Rate Cooling100
Section II. Cryopreservation Protocols for Working Laboratories116
Cryopreservation of Algae117
Cryopreservation of Bryophytes and Ferns139
Cryopreservation of Dedifferentiated Cell Cultures163
Cryopreservation of Embryogenic Cultures199
Cryopreservation of Excised Embryos and Embryonic Axes233
Cryopreservation of Monocots263
Cryopreservation of Herbaceous Dicots303
Cryopreservation of Temperate Berry Crops355
Cryopreservation of In Vitro Tissues of Deciduous Forest Trees387
Cryopreservation of Fruit and Ornamental Trees409
Cryopreservation of Dormant Buds443
Cryopreservation of Pollen465
Cryopreservation of Recalcitrant ( i. e. Desiccation- Sensitive) Seeds487
Cryopreservation of Orthodox (Desiccation Tolerant) Seeds507
Index524