| Preface | 6 |
---|
| Acknowledgements | 8 |
---|
| Contents | 9 |
---|
| Contributors | 19 |
---|
| Section I: Introduction to Cryopreservation | 28 |
---|
| Cryopreservation—Practical Considerations | 29 |
| Cryopreservation Theory | 40 |
| Development of PVS-Based Vitrification and Encapsulation– Vitrification Protocols | 58 |
| The Development of Encapsulation Dehydration | 83 |
| Controlled Rate Cooling | 100 |
| Section II. Cryopreservation Protocols for Working Laboratories | 116 |
---|
| Cryopreservation of Algae | 117 |
| Cryopreservation of Bryophytes and Ferns | 139 |
| Cryopreservation of Dedifferentiated Cell Cultures | 163 |
| Cryopreservation of Embryogenic Cultures | 199 |
| Cryopreservation of Excised Embryos and Embryonic Axes | 233 |
| Cryopreservation of Monocots | 263 |
| Cryopreservation of Herbaceous Dicots | 303 |
| Cryopreservation of Temperate Berry Crops | 355 |
| Cryopreservation of In Vitro Tissues of Deciduous Forest Trees | 387 |
| Cryopreservation of Fruit and Ornamental Trees | 409 |
| Cryopreservation of Dormant Buds | 443 |
| Cryopreservation of Pollen | 465 |
| Cryopreservation of Recalcitrant ( i. e. Desiccation- Sensitive) Seeds | 487 |
| Cryopreservation of Orthodox (Desiccation Tolerant) Seeds | 507 |
| Index | 524 |